Abstract
Measuring the functional behavior of G protein-coupled receptors (GPCRs) has been a major focus of academic and pharmaceutical research for many decades. These efforts have led to the development of many assays to measure the downstream effects of ligand binding on receptor activity. In this chapter, we describe an internalization/recycling assay that can be used to track changes in receptor number at the plasma membrane. Used in concert with other assays, this antibody-based technique can provide important information on GPCR activation by receptor-specific ligands.
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Acknowledgements
Supported by National Institutes of Health (grants DA011322 and DA021696).
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Wager-Miller, J., Mackie, K. (2016). Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_26
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DOI: https://doi.org/10.1007/978-1-4939-3539-0_26
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