Abstract
Cytochrome P450 enzymes are a large family of heme-containing proteins that have important functions in the biotransformation of xenobiotics, including pharmacologic and environmental agents, as well as of endogenously produced chemicals with broad structural and functional diversity. Anandamide and 2-arachidonoylglycerol (2-AG) are substrates for P450s expressed in multiple tissues, leading to the production of a diverse set of mono- and di-oxygenated metabolites. This chapter describes tools and methods that have been used to identify major endocannabinoid-metabolizing P450s and their corresponding products, by using subcellular tissue fractions, cultured cells, and purified recombinant enzymes in a reconstituted system.
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Acknowledgements
This work was supported in part by the National Institutes of Health (Grants CA16954 and GM007767).
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Snider, N.T., Walker, V.J., Hollenberg, P.F. (2016). Assay of Endocannabinoid Oxidation by Cytochrome P450. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_23
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DOI: https://doi.org/10.1007/978-1-4939-3539-0_23
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