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Assay of NAPE-PLD Activity

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1412))

Abstract

N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).

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References

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Acknowledgements

This study was supported by the Italian Ministry of Education, University and Research (Ministero dell’Istruzione, dell’Università e della Ricerca, grant PRIN 2010–2011 to M.M., and grant PRIN 2012 to F.F.).

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Authors and Affiliations

  1. Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy

    Filomena Fezza

  2. Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy

    Nicolina Mastrangelo & Mauro Maccarrone

  3. European Center for Brain Research/IRCCS Santa Lucia Foundation, Rome, Italy

    Mauro Maccarrone

Authors
  1. Filomena Fezza
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  2. Nicolina Mastrangelo
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  3. Mauro Maccarrone
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Corresponding author

Correspondence to Filomena Fezza .

Editor information

Editors and Affiliations

  1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

    Mauro Maccarrone

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Fezza, F., Mastrangelo, N., Maccarrone, M. (2016). Assay of NAPE-PLD Activity. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3539-0_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3537-6

  • Online ISBN: 978-1-4939-3539-0

  • eBook Packages: Springer Protocols

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