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Superresolution Microscopy of the Nuclear Envelope and Associated Proteins

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The Nuclear Envelope

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1411))

Abstract

Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.

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Acknowledgments

Srivats Hariharan (Olympus Singapore, formerly IMB, A*STAR) for building the SMLM microscope in the IMB Microscopy Unit together with the lab of Sohail Ahmed (IMB, A*STAR), John Lim Soon Yew (IMB, A*STAR) for assisting with the maintenance and operation of the SMLM microscope and for supporting the image processing and analysis, Declan Lunny (IMB, A*STAR) for the advice and help with the sample preparation, and Brian Burke (IMB, A*STAR) and Colin Stewart (IMB, A*STAR) for supervising the research projects within their labs.

Author information

Authors and Affiliations

  1. Institute of Medical Biology, A*STAR, 8A Biomedical Grove, #06-06 Immunos, Singapore, 138648, Singapore

    Wei Xie & Graham D. Wright

  2. College of Science and Engineering, Qatar Foundation, Hamad bin Khalifa University, 5825, Doha, Qatar

    Henning F. Horn

Authors
  1. Wei Xie
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  2. Henning F. Horn
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  3. Graham D. Wright
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Corresponding author

Correspondence to Graham D. Wright .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Leicester, Leicester, United Kingdom

    Sue Shackleton

  2. Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

    Philippe Collas

  3. Wellcome Trust Centre for Cell Biol, University of Edinburgh, Edinburgh, United Kingdom

    Eric C. Schirmer

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Xie, W., Horn, H.F., Wright, G.D. (2016). Superresolution Microscopy of the Nuclear Envelope and Associated Proteins. In: Shackleton, S., Collas, P., Schirmer, E. (eds) The Nuclear Envelope. Methods in Molecular Biology, vol 1411. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3530-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3530-7_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3528-4

  • Online ISBN: 978-1-4939-3530-7

  • eBook Packages: Springer Protocols

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