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Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells

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The Nuclear Envelope

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1411))

Abstract

Skeletal muscle cells are large syncytia, containing hundreds of nuclei positioned regularly along the length of the fiber. During development, nuclei are actively distributed throughout the myotube by the microtubule motor proteins, kinesin-1, and cytoplasmic dynein. Nuclear movement consists of translocation along the long axis of the cell concurrent with three-dimensional rotation of nuclei. In this chapter we describe methods for quantitatively assessing the speed of nuclear rotation in cultured myotubes using live-cell imaging techniques coupled with rigid body kinematic analyses. Additionally, we provide protocols for analyzing nuclear distribution in myotubes.

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Acknowledgments

This work was supported by the National Institutes of Health (P01 GM087253 to E.L.F.H., T32 GM-07229, and T32 AR-053461 to M.H.W.) and the American Heart Association (#13PRE16090007 to M.H.W.).

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Authors and Affiliations

  1. Department of Physiology, The Pennsylvania Muscle Institute, Perelman School of Medicine at the University of Pennsylvania, 638A Clinical Research Building, 415 Curie Boulevard, Philadelphia, PA, 19104-6085, USA

    Meredith H. Wilson & Erika L. F. Holzbaur

  2. Space Exploration Sector, Johns Hopkins Applied Physics Laboratory, Laurel, MD, 21723, USA

    Matthew G. Bray

Authors
  1. Meredith H. Wilson
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  2. Matthew G. Bray
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  3. Erika L. F. Holzbaur
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Corresponding author

Correspondence to Erika L. F. Holzbaur .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of Leicester, Leicester, United Kingdom

    Sue Shackleton

  2. Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway

    Philippe Collas

  3. Wellcome Trust Centre for Cell Biol, University of Edinburgh, Edinburgh, United Kingdom

    Eric C. Schirmer

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Wilson, M.H., Bray, M.G., Holzbaur, E.L.F. (2016). Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells. In: Shackleton, S., Collas, P., Schirmer, E. (eds) The Nuclear Envelope. Methods in Molecular Biology, vol 1411. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3530-7_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3530-7_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3528-4

  • Online ISBN: 978-1-4939-3530-7

  • eBook Packages: Springer Protocols

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