In targeted proteomics, the development of robust methodologies is dependent upon the selection of a set of optimal peptides for each protein-of-interest. Unfortunately, predicting which peptides and respective product ion transitions provide the greatest signal-to-noise ratio in a particular assay matrix is complicated. Using in vitro synthesized proteins as analytical standards, we report here an empirically driven method for the selection of said peptides in a human plasma assay matrix.
Targeted proteomics Selected reaction monitoring In vitro translation Human plasma proteome Proteotypic peptides
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This work was supported in part by National Institutes of Health grants P41 GM103533, R01 GM107142, and R01 GM107806.
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