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Reversible Photoregulation of Gene Expression and Translation

  • Shinzi OgasawaraEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1408)

Abstract

Several methods for controlling gene expression by light illumination have been reported. Most of these methods control transcription by regulating the interaction between DNA and transcription factors. The use of a photolabile protecting compound (cage compound) is another promising approach for controlling gene expression, although typically in an irreversible manner. We here describe a new approach for reversibly controlling translation using a photoresponsive 8-styryl cap (8ST-cap) that can be reversibly isomerized by illumination with light of a specific wavelength.

Key words

mRNA Translation Cap structure Photoisomerization 

Notes

Acknowledgments

The present work was supported by Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST) and JSPS KAKENHI Grant Number 25104526.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Creative Research Institution Sousei (CRIS)Hokkaido UniversitySapporoJapan

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