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Optogenetics pp 377-387 | Cite as

Optogenetic Manipulation of Selective Neural Activity in Free-Moving Drosophila Adults

  • Po-Yen Hsiao
  • Ming-Chin Wu
  • Yen-Yin Lin
  • Chein-Chung Fu
  • Ann-Shyn ChiangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1408)

Abstract

Activating selected neurons elicits specific behaviors in Drosophila adults. By combining optogenetics and laser-tracking techniques, we have recently developed an automated laser-tracking and optogenetic manipulation system (ALTOMS) for studying how brain circuits orchestrate complex behaviors. The established ALTOMS can independently target three lasers (473-nm blue laser, 593.5-nm yellow laser, and 1064-nm infrared laser) on any specified body part of two freely moving flies. Triggering light-sensitive proteins in real time, the blue laser and yellow laser can respectively activate and inhibit target neurons in artificial transgenic flies. Since infrared light is invisible to flies, we use the 1064-nm laser as an aversive stimulus in operant learning without perturbing visual inputs. Herein, we provide a detailed protocol for the construction of ALTOMS and optogenetic manipulation of target neurons in Drosophila adults during social interactions.

Key words

Optogenetics Channelrhodopsin Halorhodopsin Optical stimulation Neural circuit Freely walking Behavior Drosophila 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Po-Yen Hsiao
    • 1
  • Ming-Chin Wu
    • 2
  • Yen-Yin Lin
    • 2
    • 3
    • 4
  • Chein-Chung Fu
    • 3
    • 5
    • 6
  • Ann-Shyn Chiang
    • 1
    • 2
    • 7
    • 8
    Email author
  1. 1.Institute of BiotechnologyNational Tsing Hua UniversityHsinchuTaiwan
  2. 2.Brain Research CenterNational Tsing Hua UniversityHsinchuTaiwan
  3. 3.Institute of Photonics TechnologiesNational Tsing Hua UniversityHsinchuTaiwan
  4. 4.Department of Electrical EngineeringNational Tsing Hua UniversityHsinchuTaiwan
  5. 5.Department of Power Mechanical EngineeringNational Tsing Hua UniversityHsinchuTaiwan
  6. 6.Institute of Nanotechnology and Microsystems EngineeringNational Tsing Hua UniversityHsinchuTaiwan
  7. 7.Genomics Research CenterAcademia SinicaTaipeiTaiwan
  8. 8.Kavli Institute for Brain and MindUniversity of California, San DiegoLa JollaUSA

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