Optogenetics pp 345-362 | Cite as

Optogenetic Control of Fibroblast Growth Factor Receptor Signaling

  • Nury Kim
  • Jin Man Kim
  • Won Do HeoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1408)


FGFR1 is a member of the fibroblast growth factor family, which controls diverse cellular functions such as cell proliferation, migration, and differentiation. OptoFGFR1, an optogenetic method to modulate the FGFR signaling pathway with light by utilizing PHR domain of cryptochrome2 and cytoplasmic region of FGFR1, enabled light-guided activation of FGFR to study its effects on downstream signaling pathway and during diverse biological processes such as cell migration. Here, we describe about optogenetic and microscopic methods to spatiotemporally manipulate FGFR signaling in a single cell or group of cells using confocal microscope and LED array.

Key words

FGFR optoFGFR1 Optogenetics Spatiotemporal regulation Signaling pathway Migration Live cell imaging 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Center for Cognition and SocialityInstitute for Basic ScienceSeoulRepublic of Korea
  2. 2.Graduate School of Medical Science and EngineeringKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea
  3. 3.Department of Biological SciencesKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea

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