Optogenetic Tools for Confined Stimulation in Deep Brain Structures
Optogenetics has emerged in the past decade as a technique to modulate brain activity with cell-type specificity and with high temporal resolution. Among the challenges associated with this technique is the difficulty to target a spatially restricted neuron population. Indeed, light absorption and scattering in biological tissues make it difficult to illuminate a minute volume, especially in the deep brain, without the use of optical fibers to guide light. This work describes the design and the in vivo application of a side-firing optical fiber adequate for delivering light to specific regions within a brain subcortical structure.
Key wordsDeep brain stimulation Optogenetics Side-firing optical fiber
Supported by a Fonds de Recherche du Québec-Nature et Technologies (FRQ-NT) grant #165075 (Projet de recherche en équipe) to F.L. and C.C. and by Natural Sciences and Engineering Research Council of Canada (NSERC) grants #194670 and 239876 to C.C. and F.L., respectively. A.C. was supported in part by scholarships from the Fonds de Recherche en Santé du Québec (FRSQ) vision network and the Faculté des Études Supérieures et Postdoctorales-Institut de Génie Biomedical (FESP-IGB) of the Université de Montréal.
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