Abstract
Optogenetics is rapidly gaining acceptance as a preferred method to study specific neuronal cell types using light. Optogenetic neuromodulation requires the introduction of a cell-specific viral vector encoding for a light activating ion channel or ion pump and the utilization of a system to deliver light stimulation to brain. Here, we describe a two-part methodology starting with a procedure to inject an optogenetic AAV virus into rat cortex followed by a second procedure to surgically implant an optical cannula for light delivery to the deeper cortical layers.
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Acknowledgement
This work was supported by National Institutes of Health (NIBIB R01-EB000215NIH) and the Advancing a Healthier Wisconsin Program (5520208). R. Pashaie is supported by National Science Foundation (NSF) Career Award grant # MIL109784.
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Pawela, C., DeYoe, E., Pashaie, R. (2016). Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex. In: Kianianmomeni, A. (eds) Optogenetics. Methods in Molecular Biology, vol 1408. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3512-3_15
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DOI: https://doi.org/10.1007/978-1-4939-3512-3_15
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Publisher Name: Humana Press, New York, NY
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