Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
The technology of optogenetics provides a new method to modulate neural activity with spatial specificity and millisecond-temporal scale. This nonelectrical modulation method also gives chance for simultaneous electrophysiological recording during stimulations. Here, we describe our locomotor activity modulation on free-behaving rats using optogenetic techniques. The target sites of the rat brain were dorsal periaqueductal gray (dPAG) and ventral tegmental area (VTA) for the modulation of defensive and reward behaviors, respectively.
Key wordsOptogenetics Locomotor activity modulation Optical-electrode array dPAG VTA Virus injection
We thank Dr. Sicong Chen and Chaonan Yu for providing part technical supports. This work was supported by (1) International Science & Technology Cooperation Program of China, 2014DFG32580; (2) National Natural Science Foundation of China, 61305145; (3) Specialized Research Fund for Doctoral Program of Higher Education, 20130101120166; (4) Fundamental Research Funds for the Central Universities.
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