Optogenetics pp 195-206 | Cite as

Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats

  • Kedi Xu
  • Jiacheng Zhang
  • Songchao Guo
  • Xiaoxiang ZhengEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1408)


The technology of optogenetics provides a new method to modulate neural activity with spatial specificity and millisecond-temporal scale. This nonelectrical modulation method also gives chance for simultaneous electrophysiological recording during stimulations. Here, we describe our locomotor activity modulation on free-behaving rats using optogenetic techniques. The target sites of the rat brain were dorsal periaqueductal gray (dPAG) and ventral tegmental area (VTA) for the modulation of defensive and reward behaviors, respectively.

Key words

Optogenetics Locomotor activity modulation Optical-electrode array dPAG VTA Virus injection 



We thank Dr. Sicong Chen and Chaonan Yu for providing part technical supports. This work was supported by (1) International Science & Technology Cooperation Program of China, 2014DFG32580; (2) National Natural Science Foundation of China, 61305145; (3) Specialized Research Fund for Doctoral Program of Higher Education, 20130101120166; (4) Fundamental Research Funds for the Central Universities.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kedi Xu
    • 1
    • 2
    • 3
  • Jiacheng Zhang
    • 1
    • 2
    • 3
  • Songchao Guo
    • 1
    • 2
    • 3
  • Xiaoxiang Zheng
    • 1
    • 2
    • 3
    Email author
  1. 1.Qiushi Academy for Advanced Studies (QAAS)Zhejiang UniversityHangzhouChina
  2. 2.Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education MinistryZhejiang UniversityHangzhouChina
  3. 3.Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness AppraisalHangzhouChina

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