Advertisement

Optogenetics pp 195-206 | Cite as

Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats

  • Kedi Xu
  • Jiacheng Zhang
  • Songchao Guo
  • Xiaoxiang ZhengEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1408)

Abstract

The technology of optogenetics provides a new method to modulate neural activity with spatial specificity and millisecond-temporal scale. This nonelectrical modulation method also gives chance for simultaneous electrophysiological recording during stimulations. Here, we describe our locomotor activity modulation on free-behaving rats using optogenetic techniques. The target sites of the rat brain were dorsal periaqueductal gray (dPAG) and ventral tegmental area (VTA) for the modulation of defensive and reward behaviors, respectively.

Key words

Optogenetics Locomotor activity modulation Optical-electrode array dPAG VTA Virus injection 

Notes

Acknowledgements

We thank Dr. Sicong Chen and Chaonan Yu for providing part technical supports. This work was supported by (1) International Science & Technology Cooperation Program of China, 2014DFG32580; (2) National Natural Science Foundation of China, 61305145; (3) Specialized Research Fund for Doctoral Program of Higher Education, 20130101120166; (4) Fundamental Research Funds for the Central Universities.

References

  1. 1.
    Umemura A, Oyama G, Shimo Y et al (2013) Update on deep brain stimulation for movement disorders. Rinsho Shinkeigaku 53:911–914CrossRefPubMedGoogle Scholar
  2. 2.
    Talwar SK, Xu S, Hawley ES et al (2002) Behavioural neuroscience: rat navigation guided by remote control. Nature 417(6884):37–38CrossRefPubMedGoogle Scholar
  3. 3.
    Feng Z, Chen W, Ye X et al (2007) A remote control training system for rat navigation in complicated environment. J Zhejiang Univ Sci A 8(2):323–330CrossRefGoogle Scholar
  4. 4.
    Lim LW, Blokland A, Visser-Vandewalle V et al (2008) High-frequency stimulation of the dorsolateral periaqueductal gray and ventromedial hypothalamus fails to inhibit panic-like behaviour. Behav Brain Res 193(2):197–203CrossRefPubMedGoogle Scholar
  5. 5.
    Brandao ML, Anseloni VZ, Pandossio JE et al (1999) Neurochemical mechanisms of the defensive behavior in the dorsal midbrain. Neurosci Biobehav Rev 23(6):863–875CrossRefPubMedGoogle Scholar
  6. 6.
    McIntyre CC, Mori S, Sherman DL et al (2004) Electric field and stimulating influence generated by deep brain stimulation of the subthalamic nucleus. Clin Neurophysiol 115(3):589–595CrossRefPubMedGoogle Scholar
  7. 7.
    Deisseroth K, Feng G, Majewska AK et al (2006) Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci 26(41):10380–10386CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Chen S, Zhou H, Guo S et al (2015) Optogenetics based rat–robot control: optical stimulation encodes “stop” and “escape” commands. Ann Biomed Eng 43(8):1851–1864CrossRefPubMedGoogle Scholar
  9. 9.
    Guo SC, Zhou H, Wang YM et al (2014) A rat-robot control system based on optogenetics. Appl Mech Mater 461:848–852CrossRefGoogle Scholar
  10. 10.
    Guo S, Chen S, Zhang Q et al (2014) Optogenetic activation of the excitatory neurons expressing CaMKIIα in the ventral tegmental area upregulates the locomotor activity of free behaving rats. Biomed Res Int 2014:687469PubMedPubMedCentralGoogle Scholar
  11. 11.
    Guo S, Zhou H, Zhang J et al (2013) A multi-electrode array coupled with fiberoptic for deep-brain optical neuromodulation and electrical recording. In: Engineering in Medicine and Biology Society (EMBC), 2013 35th annual international conference of the IEEE. pp 2752–2755Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kedi Xu
    • 1
    • 2
    • 3
  • Jiacheng Zhang
    • 1
    • 2
    • 3
  • Songchao Guo
    • 1
    • 2
    • 3
  • Xiaoxiang Zheng
    • 1
    • 2
    • 3
    Email author
  1. 1.Qiushi Academy for Advanced Studies (QAAS)Zhejiang UniversityHangzhouChina
  2. 2.Department of Biomedical Engineering, Key Laboratory of Biomedical Engineering of Education MinistryZhejiang UniversityHangzhouChina
  3. 3.Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness AppraisalHangzhouChina

Personalised recommendations