Skip to main content
SpringerLink
Log in

Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D

  • Protocol
  • First Online:
Chemotaxis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1407))

Abstract

We describe three chemotaxis assays—Insall chambers, circular invasion assays, and 3D organotypic assays—that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Insall R (2013) The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration. Curr Opin Cell Biol 25:526–531

    Article  CAS  PubMed  Google Scholar 

  2. Roussos ET, Condeelis JS, Patsialou A (2011) Chemotaxis in cancer. Nat Rev Cancer 11:573–587

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zigmond SH, Hirsch JG (1973) Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med 137:387–410

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Paguirigan AL, Beebe DJ (2008) Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays. Bioessays 30:811–821

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Zigmond SH (1974) Mechanisms of sensing chemical gradients by polymorphonuclear leukocytes. Nature 249:450–452

    Article  CAS  PubMed  Google Scholar 

  6. Zicha D, Dunn GA, Brown AF (1991) A new direct-viewing chemotaxis chamber. J Cell Sci 99:769–775

    PubMed  Google Scholar 

  7. Muinonen-Martin AJ, Knecht DA, Veltman DM et al (2013) Measuring chemotaxis using direct visualization microscope chambers. Methods Mol Biol 1046:307–321

    Article  PubMed  Google Scholar 

  8. Timpson P, McGhee EJ, Erami Z et al (2011) Organotypic collagen I assay: a malleable platform to assess cell behaviour in a 3-dimensional context. J Vis Exp 56:3089

    Google Scholar 

  9. Yu X, Machesky LM (2012) Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay. PLoS One 7:e30605

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Muinonen-Martin AJ, Susanto O, Zhang Q et al (2014) Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biol 12:e1001966

    Article  PubMed  PubMed Central  Google Scholar 

  11. Santiago-Walker A, Li L, Haass NK, Herlyn M (2009) Melanocytes: from morphology to application. Skin Pharmacol Physiol 22:114–121

    Article  CAS  PubMed  Google Scholar 

  12. Berking C, Herlyn M (2001) Human skin reconstruct models: a new application for studies of melanocyte and melanoma biology. Histol Histopathol 16:669–674

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK

    Olivia Susanto, Max Nobis & Robert H. Insall

  2. Melanoma Clinic, Leeds Cancer Centre, St. James’s University Hospital, Beckett Street, Leeds, LS9 7TF, UK

    Andrew J. Muinonen-Martin

Authors
  1. Olivia Susanto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew J. Muinonen-Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Max Nobis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert H. Insall
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Robert H. Insall .

Editor information

Editors and Affiliations

  1. Nat Inst of Allergy& Infectious Dis, NIH, Laboratory of Immunogenetics, Rockville, Maryland, USA

    Tian Jin

  2. National Institutes of Health, Nat Inst Alcohol Abuse & Alcoholism, Rockville, Maryland, USA

    Dale Hereld

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Susanto, O., Muinonen-Martin, A.J., Nobis, M., Insall, R.H. (2016). Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D. In: Jin, T., Hereld, D. (eds) Chemotaxis. Methods in Molecular Biology, vol 1407. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3480-5_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3480-5_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3478-2

  • Online ISBN: 978-1-4939-3480-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation