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Production of Aromatic Plant Terpenoids in Recombinant Baker’s Yeast

  • Anita Emmerstorfer-AugustinEmail author
  • Harald Pichler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1405)

Abstract

Plant terpenoids are high-value compounds broadly applied as food additives or fragrances in perfumes and cosmetics. Their biotechnological production in yeast offers an attractive alternative to extraction from plants. Here, we provide two optimized protocols for the production of the plant terpenoid trans-nootkatol with recombinant S. cerevisiae by either (I) converting externally added (+)-valencene with resting cells or (II) cultivating engineered self-sufficient production strains. By synthesis of the hydrophobic compounds in self-sufficient production cells, phase transfer issues can be avoided and the highly volatile products can be enriched in and easily purified from n-dodecane, which is added to the cell broth as second phase.

Key words

Plant terpenoids Yeast S. cerevisiae Cytochrome P450 enzymes Biotransformation (+)-Valencene synthase tHMG1 Metabolic engineering 

Notes

Acknowledgements

We thank Prof. Erich Leitner for providing help with GC-FID measurements. We also thank Martin Schürmann, Iwona Kaluzna, Monika Müller, Andreas Kolb, and Daniel Mink (DSM Chemical Technology R and D) for fruitful discussion. This work has been supported by the Federal Ministry of Science, Research and Economy (BMWFW), the Federal Ministry of Traffic, Innovation and Technology (bmvit), the Styrian Business Promotion Agency SFG, and the Standortagentur Tiroland ZIT—Technology Agency of the City of Vienna through the COMET—Funding Program managed by the Austrian Research Promotion Agency FFG.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.ACIB - Austrian Centre of Industrial BiotechnologyGrazAustria
  2. 2.Institute of Molecular BiotechnologyNAWI Graz, Graz University of TechnologyGrazAustria

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