Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves

  • Svend Roesen Madsen
  • Hussam Hassan Nour-Eldin
  • Barbara Ann HalkierEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1405)


The leaf apoplast comprises the extracellular continuum outside cell membranes. A broad range of processes take place in the apoplast, including intercellular signaling, metabolite transport, and plant-microbe interactions. To study these processes, it is essential to analyze the metabolite content in apoplastic fluids. Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described.

Key words

Apoplastic fluid Guttation fluid Vacuum infiltration Glucosinolates 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Svend Roesen Madsen
    • 1
  • Hussam Hassan Nour-Eldin
    • 1
  • Barbara Ann Halkier
    • 1
    Email author
  1. 1.DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of ScienceUniversity of CopenhagenFrederiksberg CDenmark

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