RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content.
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
Lorenz WW, Yu Y-C, Dean JFD (2010) An improved method of RNA isolation from Loblolly Pine (P. taeda L.) and other conifer species. J Vis Exp 36:1751–1756PubMedGoogle Scholar
MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226CrossRefGoogle Scholar
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal Biochem 162:156–159CrossRefPubMedGoogle Scholar
Ding LW, Sun QY, Wang ZY, Sun YB, Xu ZF (2008) Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate. Anal Biochem 374:426–428CrossRefPubMedGoogle Scholar
Camacho-Villasana YM, Ochoa-Alejo N, Walling L, Bray EA (2002) An improved method for isolating RNA from dehydrated and non- dehydrated Chili pepper (Capsicum annuum L.) plant tissues. Pl Mol Biol Rep 20:407–414CrossRefGoogle Scholar
Liao Z, Chen M, Guo L, Gong Y, Tang F, Sun X, Tang K (2004) Rapid isolation of high-quality total RNA from Taxus and Ginkgo. Prep Biochem Biotechnol 34:209–214CrossRefPubMedGoogle Scholar
Loomis MD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol 31:528–544CrossRefPubMedGoogle Scholar