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A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines

  • Júlio César de Lima
  • Thanise Nogueira Füller
  • Fernanda de Costa
  • Kelly C. S. Rodrigues-Corrêa
  • Arthur G. Fett-NetoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1405)

Abstract

RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content.

Key words

RNA Pines Concert plant RNA reagent Stem RNA extraction Oleoresin Conifers 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Júlio César de Lima
    • 1
  • Thanise Nogueira Füller
    • 2
    • 3
  • Fernanda de Costa
    • 5
    • 6
  • Kelly C. S. Rodrigues-Corrêa
    • 4
  • Arthur G. Fett-Neto
    • 2
    • 3
    Email author
  1. 1.Molecular Genetics LaboratoryUniversity of Passo FundoPasso FundoBrazil
  2. 2.Plant Physiology Laboratory, Center for BiotechnologyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  3. 3.Department of BotanyUFRGSPorto AlegreBrazil
  4. 4.Regional Integrated University of Alto Uruguai and Missões (URI-FW)Frederico WestphalenBrazil
  5. 5.Plant Physiology Laboratory, Department of BotanyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  6. 6.Center for BiotechnologyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil

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