Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction

  • Thanise Nogueira Füller
  • Júlio César de Lima
  • Fernanda de Costa
  • Kelly C. S. Rodrigues-Corrêa
  • Arthur G. Fett-NetoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1405)


Tapping technique comprises the extraction of pine oleoresin, a non-wood forest product consisting of a complex mixture of mono, sesqui, and diterpenes biosynthesized and exuded as a defense response to wounding. Oleoresin is used to produce gum rosin, turpentine, and their multiple derivatives. Oleoresin yield and quality are objects of interest in pine tree biotechnology, both in terms of environmental and genetic control. Monitoring these parameters in individual trees grown in the field provides a means to examine the control of terpene production in resin canals, as well as the identification of genetic-based differences in resinosis. A typical method of tapping involves the removal of bark and application of a chemical stimulant on the wounded area. Here we describe the methods for preparing the resin-stimulant paste with different adjuvants, as well as the bark streaking process in adult pine trees.

Key words

Oleoresin Pine Tapping Chemical stimulant Wounding 


  1. 1.
    Rodrigues-Corrêa KCS, Fett-Neto AG (2012) Physiological control of pine resin production. In: Fett-Neto AG, Rodrigues-Corrêa KCS (eds) Pine resin: biology, chemistry and applications. Research Signpost, Kerala, p 25Google Scholar
  2. 2.
    Rodrigues-Corrêa KCS, de Lima JC, Fett-Neto AG (2012) Pine oleoresin: tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees. Food Energy Secur 1:81–93CrossRefGoogle Scholar
  3. 3.
    Rodrigues KCS, Apel MA, Henriques AT, Fett-Neto AG (2011) Efficient oleoresin biomass production in pines using low cost metal containing stimulant paste. Biomass Bioenergy 3:4442–4448CrossRefGoogle Scholar
  4. 4.
    Coppen J, Hone GA (1995) Gum naval stores: naval stores: turpentine and rosin from pine resin. Non-wood forest products, vol 2. FAO, Rome, 63Google Scholar
  5. 5.
    Kossuth SV, Koch P (1989) Paraquat and CEPA stimulation of oleoresin production in lodgepole pine central stump-root system. Wood Fiber Sci 21:263–273Google Scholar
  6. 6.
    Wolter KE, Zinkel DF (1984) Observations on the physiological oleoresin synthesis in Pinus resinosa. Can J For Res 14:452–458CrossRefGoogle Scholar
  7. 7.
    De Geyter N, Gholami A, Goormachtig S, Goossens A (2012) Transcriptional machineries in jasmonate-elicited plant secondary metabolism. Trends Plant Sci 17:349–359CrossRefPubMedGoogle Scholar
  8. 8.
    Hudgins JW, Christiansen E, Franceschi VR (2004) Induction of anatomically based defense responses in stems of diverse conifers by methyl jasmonate: a phylogenetic perspective. Tree Physiol 24:251–264CrossRefPubMedGoogle Scholar
  9. 9.
    Hudgins JW, Franceschi VR (2004) Methyl jasmonate-induced ethylene production is responsible for conifer phloem defense responses and reprogramming of stem cambial zone for traumatic resin duct formation. Plant Physiol 135:1–16CrossRefGoogle Scholar
  10. 10.
    Rodrigues KCS, Fett-Neto AG (2009) Oleoresin yield of Pinus elliottii in a subtropical climate: seasonal variation and effect of auxin and salicylic acid based stimulant paste. Ind Crop Prod 30:316–320CrossRefGoogle Scholar
  11. 11.
    Rodrigues KCS, Azevedo PC, Sobreiro LE, Pelissari P, Fett-Neto AG (2008) Oleoresin yield of Pinus elliottii plantations in a subtropical climate: effect of tree diameter, wound shape and concentration of active adjuvants in resin stimulating paste. Ind Crop Prod 27:322–327CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Thanise Nogueira Füller
    • 1
    • 2
  • Júlio César de Lima
    • 3
  • Fernanda de Costa
    • 4
    • 5
  • Kelly C. S. Rodrigues-Corrêa
    • 6
  • Arthur G. Fett-Neto
    • 1
    • 2
    Email author
  1. 1.Plant Physiology Laboratory, Center for BiotechnologyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Department of BotanyUFRGSPorto AlegreBrazil
  3. 3.Molecular Genetics LaboratoryUniversity of Passo FundoPasso FundoBrazil
  4. 4.Plant Physiology Laboratory, Department of BotanyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  5. 5.Center for BiotechnologyFederal University of Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  6. 6.Regional Integrated University of Alto Uruguai and Missões (URI-FW)Frederico WestphalenBrazil

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