Protoplast Transformation as a Plant-Transferable Transient Expression System
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The direct uptake of DNA by naked plant cells (protoplasts) provides an expression system of exception for the quickly growing research in non-model plants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials.
Key wordsPlant-transferable methodology PEG-mediated transformation Protoplasts Transient expression
This work was supported by: (1) Fundo Europeu de Desenvolvimento Regional funds through the Operational Competitiveness Programme COMPETE and by National Funds through Fundação para a Ciência e a Tecnologia (FCT) under the projects FCOMP-01-0124-FEDER-037277 (PEst-C/SAU/LA0002/2013), FCOMP-01-0124-FEDER-019664 (PTDC/BIA-BCM/119718/2010) and FCOMP-01-0124-FEDER-028125 (PTDC/BBB-BIO/2231/2012); (2) by the FCT scholarships co-supported by FCT and POPH-QREN (European Social Fund) SFRH/BD/41907/2007 (IC), SFRH/BD/48283/2008 (SB) and SFRH/BPD/20669/2004 (PD); (3) by a Scientific Mecenate Grant from Grupo Jerónimo Martins.
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