A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes

  • Morten Egevang Jørgensen
  • Hussam Hassan Nour-Eldin
  • Barbara Ann HalkierEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1405)


Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.

Key words

Transport protein quantification Single oocyte Western blot Xenopus oocytes 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Morten Egevang Jørgensen
    • 1
  • Hussam Hassan Nour-Eldin
    • 1
  • Barbara Ann Halkier
    • 1
    Email author
  1. 1.DynaMo Center, Department of Plant and Environmental Sciences, Faculty of ScienceUniversity of CopenhagenFrederiksberg CDenmark

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