A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
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Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.
Key wordsTransport protein quantification Single oocyte Western blot Xenopus oocytes
- 10.Galili G, Altschuler Y, Ceriotti A (1995) Synthesis of plant proteins in heterologous systems: Xenopus laevis oocytes. In: David W, Galbraith DPB, Hans JB (eds) Method cell biol. Academic, San Diego, pp 497–517Google Scholar