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Vaccine Design pp 155–166Cite as

Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization, and Protection Studies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1403))

Abstract

Rabies is a viral infection of the central nervous system for which vaccination is the only treatment possible. Besides preexposure, vaccination is highly recommended for people living in endemic areas, veterinarians, and laboratory workers. Our group has developed rabies virus-like particles (RV-VLPs) with immunogenic features expressed in mammalian cells for vaccine applications. In this chapter the methods to obtain and characterize a stable HEK293 cell line expressing RV-VLPs are detailed. Further, analytical ultracentrifugation steps to purify the obtained VLPs are developed, as well as western blot, dynamic light scattering, and immunogold electron microscopy to analyze the size, distribution, shape, and antigenic conformation of the purified particles. Finally, immunization protocols are described to study the immunogenicity of RV-VLPs.

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Acknowledgements

The authors would like to thank Juan Pablo Soto (Institutional Image, Universidad Nacional del Litoral, Argentina) for technical support.

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Authors and Affiliations

  1. Cell Culture Laboratory, Biochemistry and Biological Sciences School, Universidad Nacional del Litoral, Bv. Pellegrini 2750, Santa Fe, 3000, Argentina

    Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje & Claudio Prieto

  2. Biotechnological Development Laboratory, Biochemistry and Biological Sciences School, Universidad Nacional del Litoral, Santa Fe, Argentina

    Diego Fontana & Claudio Prieto

Authors
  1. Diego Fontana
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  2. Marina Etcheverrigaray
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  3. Ricardo Kratje
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  4. Claudio Prieto
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Corresponding author

Correspondence to Diego Fontana .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, Pennsylvania, USA

    Sunil Thomas

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Fontana, D., Etcheverrigaray, M., Kratje, R., Prieto, C. (2016). Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization, and Protection Studies. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1403. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3387-7_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3387-7_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3385-3

  • Online ISBN: 978-1-4939-3387-7

  • eBook Packages: Springer Protocols

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