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Characterization of Circular RNAs

  • Yang Zhang
  • Li YangEmail author
  • Ling-Ling ChenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1402)

Abstract

Accumulated lines of evidence reveal that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5′ cap and 3′ tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus requiring specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs derived from intron lariats (ciRNAs) and circular RNAs produced from back spliced exons (circRNAs) in human.

Key words

Circular RNAs ciRNAs circRNAs RNA fractionation RNase R 

Notes

Acknowledgements

This work was supported by 31271390, 31322018, 91440202 from NSFC to L.Y. and L.L.C.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina
  2. 2.Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, CAS Center for Excellence in Brain Science, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina
  3. 3.School of Life Science and TechnologyShanghaiTech UniversityShanghaiChina

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