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Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum higginsianum

  • Kei Hiruma
  • Yusuke SaijoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1398)

Abstract

After Colletotrichum storage methods in Chapter  23, we describe here experimental methods for the inoculation of Colletotrichum higginsianum (C.h.) on Arabidopsis leaves. We put a particular focus on the methods for lesion measurements after the drop-inoculation of the leaves and on C.h. biomass measurements in the leaves by RT-qPCR analysis. As an option, we also briefly describe methods for counting C.h. entry ratio.

Key words

Colletotrichum higginsianum Arabidopsis Lesion measurements Biomass measurements RT-qPCR 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Graduate School of Biological SciencesNara Institute of Science and TechnologyIkomaJapan
  2. 2.JST, Precursory Research for Embryonic Science and TechnologyChiyoda-kuJapan

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