Assessing Gravitropic Responses in Arabidopsis
Arabidopsis thaliana was the first higher organism to have its genome sequenced and is now widely regarded as the model dicot. Like all plants, Arabidopsis develops distinct growth patterns in response to different environmental stimuli. This can be seen in the gravitropic response of roots. Methods to investigate this particular tropism are presented here. First, we describe a high-throughput time-lapse photographic analysis of root growth and curvature response to gravistimulation allowing the quantification of gravitropic kinetics and growth rate at high temporal resolution. Second, we present a protocol that allows a quantitative evaluation of gravitropic sensitivity using a homemade 2D clinostat. Together, these approaches allow an initial comparative analysis of the key phenomena associated with root gravitropism between different genotypes and/or accessions.
Key wordsArabidopsis thaliana Gravitropism Gravity perception Time-lapse photography Clinostat
- 3.Takahashi N, Yamazaki Y, Kobayashi A, Higashitani A, Takahashi H (2003) Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish. Plant Physiol 132(805):10Google Scholar
- 7.Larsen P (1962) Orthogravitropism in roots. In: Ruhland W (ed) Encyclopedia of plant physiol, Vol 17, Part 2, physiology of movements. Springer-Verlag, Berlin, pp 153–199Google Scholar
- 13.Sangari A, Ohme-Takagi M, Mitsuda N, Assadi A (2012) Automated imaging & high-throughput phenotyping, massively parallel image acquisition, analysis and modeling: mapping plant genotype-phenotype relation. Cold Spring Harbor, Cold Spring Harbor, NYGoogle Scholar