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Assessing Gravitropic Responses in Arabidopsis

  • Richard BarkerEmail author
  • Benjamin Cox
  • Logan Silber
  • Arash Sangari
  • Amir Assadi
  • Patrick Masson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1398)

Abstract

Arabidopsis thaliana was the first higher organism to have its genome sequenced and is now widely regarded as the model dicot. Like all plants, Arabidopsis develops distinct growth patterns in response to different environmental stimuli. This can be seen in the gravitropic response of roots. Methods to investigate this particular tropism are presented here. First, we describe a high-throughput time-lapse photographic analysis of root growth and curvature response to gravistimulation allowing the quantification of gravitropic kinetics and growth rate at high temporal resolution. Second, we present a protocol that allows a quantitative evaluation of gravitropic sensitivity using a homemade 2D clinostat. Together, these approaches allow an initial comparative analysis of the key phenomena associated with root gravitropism between different genotypes and/or accessions.

Key words

Arabidopsis thaliana Gravitropism Gravity perception Time-lapse photography Clinostat 

References

  1. 1.
    Galvan Ampudia C, Julkowska M, Darwish E, Gandullo J, Korver R et al (2013) Halotropism Is a response of plant roots to avoid a saline environment. Curr Biol 23:204450CrossRefGoogle Scholar
  2. 2.
    Massa G, Gilroy S (2003) Touch modulates gravity sensing to regulate the growth of primary roots of Arabidopsis thaliana. Plant J 33:43545CrossRefGoogle Scholar
  3. 3.
    Takahashi N, Yamazaki Y, Kobayashi A, Higashitani A, Takahashi H (2003) Hydrotropism interacts with gravitropism by degrading amyloplasts in seedling roots of Arabidopsis and radish. Plant Physiol 132(805):10Google Scholar
  4. 4.
    Baldwin K, Strohm A, Masson P (2013) Gravity sensing and signal transduction in vascular plant primary roots. Am J Bot 100(1):126–142CrossRefPubMedGoogle Scholar
  5. 5.
    Strohm A, Baldwin K, Masson P (2012) Molecular mechanisms of root gravity sensing and signal transduction. Wiley Interdiscip Rev Dev Biol 1:276–285CrossRefPubMedGoogle Scholar
  6. 6.
    Strohm A, Baldwin K, Masson P (2012) Multiple roles for membrane-associated protein trafficking and signaling in gravitropism. Front Plant Sci 3:274PubMedCentralCrossRefPubMedGoogle Scholar
  7. 7.
    Larsen P (1962) Orthogravitropism in roots. In: Ruhland W (ed) Encyclopedia of plant physiol, Vol 17, Part 2, physiology of movements. Springer-Verlag, Berlin, pp 153–199Google Scholar
  8. 8.
    Perbal G, Jeune B, Lefranc A, Carnero-Diaz E, Driss-Ecole D (2002) The dose-response curve of the gravitropic reaction: a re-analysis. Physiol Plant 114:336–342CrossRefPubMedGoogle Scholar
  9. 9.
    Young L, Harrison B, Murthy N, Moffatt B, Gilroy S, Masson P (2006) Adenosine kinase modulates root gravitropism and cap morphogenesis in Arabidopsis thaliana. Plant Physiol 142:564–573PubMedCentralCrossRefPubMedGoogle Scholar
  10. 10.
    Brunoud G, Wells DM, Oliva M, Larrieu A, Mirabet V, Burrow AH, Beeckman T et al (2012) A novel sensor to map auxin response and distribution at high spatio-temporal resolution. Nature 482(7383):103–106CrossRefPubMedGoogle Scholar
  11. 11.
    Brooks T, Miller N, Spalding E (2010) Plasticity of Arabidopsis root Gravitropism throughout a multidimensional condition space quantified by automated image analysis. Plant Physiol 152(1):206–216CrossRefPubMedGoogle Scholar
  12. 12.
    Wells D, French A, Naeem A, Ishaq O, Traini R, Hijazi H, Bennett M, Pridmore T (2012) Recovering the dynamics of root growth and development using novel image acquisition and analysis methods. Philos Trans R Soc B: Biol Sci 367(1595):1517–1524CrossRefGoogle Scholar
  13. 13.
    Sangari A, Ohme-Takagi M, Mitsuda N, Assadi A (2012) Automated imaging & high-throughput phenotyping, massively parallel image acquisition, analysis and modeling: mapping plant genotype-phenotype relation. Cold Spring Harbor, Cold Spring Harbor, NYGoogle Scholar
  14. 14.
    Russino A, Ascrizzi A, Popova L, Tonazzini A, Mancuso S, Mazzolai B (2013) A novel tracking tool for the analysis of plant-root tip movements. Bioinspir Biomim 8:025004CrossRefPubMedGoogle Scholar
  15. 15.
    Buer CS, Wasteneys GO, Masle J (2003) Ethylene modulates root-wave responses in Arabidopsis. Plant Physiol 132:1085–1096PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Richard Barker
    • 1
    Email author
  • Benjamin Cox
    • 2
  • Logan Silber
    • 1
  • Arash Sangari
    • 3
  • Amir Assadi
    • 3
  • Patrick Masson
    • 1
  1. 1.Laboratory of GeneticsUniversity of WisconsinMadisonUSA
  2. 2.Medical Engineering GroupMorgridge Institute for ResearchMadisonUSA
  3. 3.Department of MathematicsUniversity of WisconsinMadisonUSA

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