Assessing Gravitropic Responses in Arabidopsis

  • Richard BarkerEmail author
  • Benjamin Cox
  • Logan Silber
  • Arash Sangari
  • Amir Assadi
  • Patrick Masson
Part of the Methods in Molecular Biology book series (MIMB, volume 1398)


Arabidopsis thaliana was the first higher organism to have its genome sequenced and is now widely regarded as the model dicot. Like all plants, Arabidopsis develops distinct growth patterns in response to different environmental stimuli. This can be seen in the gravitropic response of roots. Methods to investigate this particular tropism are presented here. First, we describe a high-throughput time-lapse photographic analysis of root growth and curvature response to gravistimulation allowing the quantification of gravitropic kinetics and growth rate at high temporal resolution. Second, we present a protocol that allows a quantitative evaluation of gravitropic sensitivity using a homemade 2D clinostat. Together, these approaches allow an initial comparative analysis of the key phenomena associated with root gravitropism between different genotypes and/or accessions.

Key words

Arabidopsis thaliana Gravitropism Gravity perception Time-lapse photography Clinostat 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Richard Barker
    • 1
    Email author
  • Benjamin Cox
    • 2
  • Logan Silber
    • 1
  • Arash Sangari
    • 3
  • Amir Assadi
    • 3
  • Patrick Masson
    • 1
  1. 1.Laboratory of GeneticsUniversity of WisconsinMadisonUSA
  2. 2.Medical Engineering GroupMorgridge Institute for ResearchMadisonUSA
  3. 3.Department of MathematicsUniversity of WisconsinMadisonUSA

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