Screening Stress Tolerance Traits in Arabidopsis Cell Cultures

  • Imma Pérez-Salamó
  • Bogáta Boros
  • László SzabadosEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1398)


Screening for tolerance traits in plant cell cultures can combine the efficiency of microbial selection and plant genetics. Agrobacterium-mediated transformation can efficiently introduce cDNA library to cell suspension cultures generating population of randomly transformed microcolonies. Transformed cultures can subsequently be screened for tolerance to different stress conditions such as salinity, high osmotic, or oxidative stress conditions. cDNA inserts in tolerant cell lines can be easily identified by PCR amplification and homology search of the determined nucleotide sequences. The described methods have been tested and used to identify regulatory genes controlling salt tolerance in Arabidopsis. As cDNA libraries can be prepared from any plants, natural diversity can be explored by using extremophile plants as gene source.

Key words

In vitro selection Agrobacterium Transformation cDNA library Gene isolation 



This research was supported by Hungarian Scientific Research Fund (OTKA Grants no. K81765, NN110962), MTA-CNR collaboration grant (2013–2015), and IPA project no. HUSRB/1002/214/036. Authors acknowledge the assistance of Annamária Király.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Imma Pérez-Salamó
    • 1
  • Bogáta Boros
    • 1
  • László Szabados
    • 1
    Email author
  1. 1.Institute of Plant BiologyBiological Research CentreSzegedHungary

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