Hydrotropism: Analysis of the Root Response to a Moisture Gradient

  • Regina Antoni
  • Daniela Dietrich
  • Malcolm J. Bennett
  • Pedro L. RodriguezEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1398)


Hydrotropism is a genuine response of roots to a moisture gradient to avoid drought. An experimental system for the induction of hydrotropic root response in petri dishes was designed by pioneering groups in the field. This system uses split agar plates containing an osmolyte only in a region of the plate in order to generate a water potential gradient. Arabidopsis seedlings are placed on the MS agar plate so that their root tips are near the junction between plain MS medium and the region supplemented with the osmolyte. This elicits a hydrotropic response in Arabidopsis roots that can be measured as the root curvature angle.

Key words

Hydrotropism Water potential gradient Root curvature angle Moisture gradient Root growth Sorbitol ABA Arabidopsis 



Funding was provided by a Marie Curie Intra-European Fellowship to R.A. and grants from the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas to P.L.R. (BIO2014-52537-R) and the Biotechnology and Biological Sciences Research Council to D.D. and M.J.B.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Regina Antoni
    • 1
  • Daniela Dietrich
    • 1
  • Malcolm J. Bennett
    • 1
  • Pedro L. Rodriguez
    • 2
    Email author
  1. 1.Centre for Plant Integrative BiologyUniversity of NottinghamNottinghamUK
  2. 2.Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones CientíficasUniversidad Politécnica de ValenciaValenciaSpain

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