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Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI

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Book cover Kidney Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1397))

Abstract

Magnetic resonance imaging (MRI) is becoming important for whole-kidney assessment of glomerular morphology, both in vivo and ex vivo. MRI-based renal morphological measurements can be made in intact organs and allow direct measurements of every perfused glomerulus. Cationic ferritin (CF) is used as a superparamagnetic contrast agent for MRI. CF binds to the glomerular basement membrane after intravenous injection, allowing direct, whole-kidney measurements of glomerular number, volume, and volume distribution. Here we describe the production, testing, and use of CF as an MRI contrast agent for quantitative glomerular morphology in intact mouse, rat, and human kidneys.

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Acknowledgements

This work was funded by a grant from the NIH Diabetic Complications Consortium, The American Heart Association, The US National Institutes of Health grant NIH DK-091722, and a grant from the Hartwell Foundation.

Author information

Authors and Affiliations

  1. Department of Biology, College of Natural Sciences, University of Hawaii at Manoa, 2540 Campus Rd., Honolulu, HI, 96822, USA

    Kevin M. Bennett

  2. Department of Radiology, Washington University School of Medicine, Saint Louis, MO, USA

    Scott C. Beeman

  3. Department of Physics, College of Natural Sciences, University of Hawaii at Manoa, Honolulu, HI, USA

    Edwin J. Baldelomar

  4. School of Computing, Informatics, and Decision Systems Engineering, Arizona State University, Tempe, AZ, USA

    Min Zhang & Teresa Wu

  5. College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Honolulu, HI, USA

    Bradley D. Hann

  6. Department of Anatomy and Developmental Biology, Monash University, Melbourne, VIC, Australia

    John F. Bertram

  7. Division of Nephrology, Department of Pediatrics, University of Virginia Medical Center, Charlottesville, VA, USA

    Jennifer R. Charlton

Authors
  1. Kevin M. Bennett
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  2. Scott C. Beeman
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  3. Edwin J. Baldelomar
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  4. Min Zhang
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  5. Teresa Wu
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  6. Bradley D. Hann
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  7. John F. Bertram
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  8. Jennifer R. Charlton
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Corresponding author

Correspondence to Kevin M. Bennett .

Editor information

Editors and Affiliations

  1. Royal Hospital Melbourne Dept. Nephrology, Melbourne, Victoria, Australia

    Tim D. Hewitson

  2. Department of Nephrology, Royal Melbourne Hospital, Parkville, Melbourne, Victoria, Australia

    Edward R. Smith

  3. Department of Nephrology, Royal Melbourne Hospital, Parkville, Victoria, Australia

    Stephen G. Holt

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© 2016 Springer Science+Business Media New York

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Bennett, K.M. et al. (2016). Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3353-2_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3351-8

  • Online ISBN: 978-1-4939-3353-2

  • eBook Packages: Springer Protocols

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