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Isolation and Propagation of Rat Peritoneal Mesothelial Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 1397)

Abstract

With the development of peritoneal dialysis in many countries, there has been much interest in the cell biology of peritoneal mesothelial cells. In this chapter we describe a reliable and reproducible method for the culture of rat primary mesothelial cells (RPMCs). This chapter outlines how to isolate mesothelial cells from rat peritoneum. The subculture of primary peritoneal mesothelial cells and the characterization by immunofluorescence is also described in detail.

Key words

  • Isolation
  • Rat primary mesothelial cells
  • Subculture
  • Characterization
  • Immunofluorescence

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  • DOI: 10.1007/978-1-4939-3353-2_3
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Correspondence to Xueqing Yu .

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Zhou, Q., Yu, X. (2016). Isolation and Propagation of Rat Peritoneal Mesothelial Cells. In: Hewitson, T., Smith, E., Holt, S. (eds) Kidney Research. Methods in Molecular Biology, vol 1397. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3353-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3353-2_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3351-8

  • Online ISBN: 978-1-4939-3353-2

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