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Induction of Human Regulatory T Cells with Bacterial Superantigens

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Superantigens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1396))

Abstract

Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

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Author information

Authors and Affiliations

  1. Division of Clinical Medicine, BSMS—Brighton and Sussex Medical School, University of Sussex, Medical Research Building, Falmer, BN1 9PS, UK

    Stefano Caserta, Amanda L. Taylor & Martin J. Llewelyn

  2. Pharmacy and Biomolecular Sciences, University of Brighton, Moulsecoomb, Brighton, BN2 4GJ, UK

    Nadia Terrazzini

  3. Brighton and Sussex University Hospitals NHS Trust, Brighton, BN2 5BE, UK

    Martin J. Llewelyn

Authors
  1. Stefano Caserta
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  2. Amanda L. Taylor
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  3. Nadia Terrazzini
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  4. Martin J. Llewelyn
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Corresponding author

Correspondence to Stefano Caserta .

Editor information

Editors and Affiliations

  1. Science Department, Concordia University - Saint Paul, St. Paul, Minnesota, USA

    Amanda J. Brosnahan

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© 2016 Springer Science+Business Media New York

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Caserta, S., Taylor, A.L., Terrazzini, N., Llewelyn, M.J. (2016). Induction of Human Regulatory T Cells with Bacterial Superantigens. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3344-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3342-6

  • Online ISBN: 978-1-4939-3344-0

  • eBook Packages: Springer Protocols

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