Abstract
In vivo responses to bacterially derived superantigen-like toxins have been difficult to define due to the inherent limitations with rodent models and the relevance that the results obtained from such models may, or may not, have for human pathophysiology. Further the use of challenge doses of superantigen toxins that are lethal or supra-lethal complicates analogies to human exposures which are rarely fatal. Here, we utilize the superantigen, staphylococcal enterotoxin B, at doses that are sublethal in a swine model of toxin-induced incapacitation. Relevant dosing using an animal species for which this toxin is a true superantigen distinguishes this model.
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Marrack P, Kappler J (1990) The staphylococcal enterotoxins and their relatives. Science 248:705–711
Spaulding AR, Salgado-Pabon W, Kohler PL, Horswill AR, Leung DY et al (2013) Staphylococcal and streptococcal superantigen exotoxins. Clin Microbiol Rev 26:422–447
Papageorgiou AC, Tranter HS, Acharya KR (1998) Crystal structure of microbial superantigen staphylococcal enterotoxin B at 1.5 A resolution: implications for superantigen recognition by MHC class II molecules and T-cell receptors. J Mol Biol 277:61–79
Swaminathan S, Furey W, Pletcher J, Sax M (1992) Crystal structure of staphylococcal enterotoxin B, a superantigen. Nature 359:801–806
Christopher GW, Cieslak TJ, Pavlin JA, Eitzen EM Jr (1997) Biological warfare. A historical perspective. JAMA 278:412–417
Madsen JM (2001) Toxins as weapons of mass destruction. A comparison and contrast with biological-warfare and chemical-warfare agents. Clin Lab Med 21:593–605
Boles JW, Pitt ML, LeClaire RD, Gibbs PH, Torres E et al (2003) Generation of protective immunity by inactivated recombinant staphylococcal enterotoxin B vaccine in nonhuman primates and identification of correlates of immunity. Clin Immunol 108:51–59
Faulkner L, Cooper A, Fantino C, Altmann DM, Sriskandan S (2005) The mechanism of superantigen-mediated toxic shock: not a simple Th1 cytokine storm. J Immunol 175:6870–6877
Stiles BG, Garza AR, Ulrich RG, Boles JW (2001) Mucosal vaccination with recombinantly attenuated staphylococcal enterotoxin B and protection in a murine model. Infect Immun 69:2031–2036
Ulrich RG, Olson MA, Bavari S (1998) Development of engineered vaccines effective against structurally related bacterial superantigens. Vaccine 16:1857–1864
Bi S, Das R, Zelazowska E, Mani S, Neill R et al (2009) The cellular and molecular immune response of the weanling piglet to staphylococcal enterotoxin B. Exp Biol Med (Maywood) 234:1305–1315
van Gessel YA, Mani S, Bi S, Hammamieh R, Shupp JW et al (2004) Functional piglet model for the clinical syndrome and postmortem findings induced by staphylococcal enterotoxin B. Exp Biol Med (Maywood) 229:1061–1071
Komisar JL, Weng CF, Oyejide A, Hunt RE, Briscoe C et al (2001) Cellular and cytokine responses in the circulation and tissue reactions in the lung of rhesus monkeys (Macaca mulatta) pretreated with cyclosporin A and challenged with staphylococcal enterotoxin B. Toxicol Pathol 29:369–378
Weng CF, Komisar JL, Hunt RE, Johnson AJ, Pitt ML et al (1997) Immediate responses of leukocytes, cytokines and glucocorticoid hormones in the blood circulation of monkeys following challenge with aerosolized staphylococcal enterotoxin B. Int Immunol 9:1825–1836
Hudson LC, Seabolt BS, Odle J, Bost KL, Stahl CH et al (2013) Sublethal staphylococcal enterotoxin B challenge model in pigs to evaluate protection following immunization with a soybean-derived vaccine. Clin Vaccine Immunol 20:24–32
Odle J, Lin X, Jacobi SK, Kim SW, Stahl CH (2014) The Suckling Piglet as an Agrimedical Model for the Study of Pediatric Nutrition and Metabolism. Ann Rev Anim Biosci 2:419–434
Swindle MM, Makin A, Herron AJ, Clubb FJ Jr, Frazier KS (2012) Swine as models in biomedical research and toxicology testing. Vet Pathol 49:344–356
Council NR (2012) Nutrition requirements of swine. National Academies Press, Washington, DC
Flournoy WS, Mani S (2009) Percutaneous external jugular vein catheterization in piglets using a triangulation technique. Lab Anim 43:344–349
Wyns H, Croubels S, Demeyere K, Watteyn A, De Backer P et al (2013) Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma. Vet Immunol Immunopathol 151:28–36
Lawson S, Lunney J, Zuckermann F, Osorio F, Nelson E et al (2010) Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination. Vaccine 28:5356–5364
Worlein JM, Baker K, Bloomsmith M, Coleman K, Koban TL (2011) The Eighth Edition of the Guide for the Care and Use of Laboratory Animals (2011); Implications for Behavioral Management. Am J Primatol 73:98–98
Chosewood LC, Wilson DE (2009) Biosafety in microbiological and biomedical laboratories. U.S. Dept. of Health and Human Services, Washington, DC, pp. xxii-415
Swindle MM (2007) Swine in the laboratory: surgery, anesthesia, imaging, and experimental techniques. CRC Press, Boca Raton, FL
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Bost, K.L., Piller, K.J., Odle, J., Stahl, C.H. (2016). A Sublethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_10
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DOI: https://doi.org/10.1007/978-1-4939-3344-0_10
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