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Proteomics in Systems Biology pp 275–286Cite as

A Simple Workflow for Large Scale Shotgun Glycoproteomics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1394))

Abstract

Targeting subproteomes is a good strategy to decrease the complexity of a sample, for example in body fluid biomarker studies. Glycoproteins are proteins with carbohydrates of varying size and structure attached to the polypeptide chain, and it has been shown that glycosylation plays essential roles in several vital cellular processes, making glycosylation a particularly interesting field of study. Here, we describe a method for the enrichment of glycosylated peptides from trypsin digested proteins in human cerebrospinal fluid. We also describe how to perform the data analysis on the mass spectrometry data for such samples, focusing on site-specific identification of glycosylation sites, using user friendly open source software.

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Acknowledgements

A.C.K. is supported by the Kristian Gerhard Jebsen Foundation. H.B. is supported by the Research Council of Norway.

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Authors and Affiliations

  1. Proteomics Unit, Department of Biomedicine, University of Bergen, Bergen, Norway

    Astrid Guldbrandsen, Harald Barsnes, Ann Cathrine Kroksveen, Frode S. Berven & Marc Vaudel

  2. KG Jebsen Centre for Multiple Sclerosis Research, Department of Clinical Medicine, University of Bergen, Bergen, Norway

    Astrid Guldbrandsen, Ann Cathrine Kroksveen & Frode S. Berven

  3. KG Jebsen Center for Diabetes Research, Department of Clinical Sciences, University of Bergen, Bergen, Norway

    Harald Barsnes

  4. Norwegian Multiple Sclerosis Competence Centre, Department of Neurology, Haukeland University Hospital, Bergen, Norway

    Frode S. Berven

Authors
  1. Astrid Guldbrandsen
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  2. Harald Barsnes
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  3. Ann Cathrine Kroksveen
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  4. Frode S. Berven
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  5. Marc Vaudel
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Corresponding author

Correspondence to Marc Vaudel .

Editor information

Editors and Affiliations

  1. University of Regensburg Institute of Functional Genomics, Regensburg, Germany

    Jörg Reinders

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Guldbrandsen, A., Barsnes, H., Kroksveen, A.C., Berven, F.S., Vaudel, M. (2016). A Simple Workflow for Large Scale Shotgun Glycoproteomics. In: Reinders, J. (eds) Proteomics in Systems Biology. Methods in Molecular Biology, vol 1394. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3341-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3341-9_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3339-6

  • Online ISBN: 978-1-4939-3341-9

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