Abstract
NK cell alloreactivity mediated by donor NK cells is a fundamental therapeutic tool in HLA haplotype mismatched hematopoietic transplantation in adult acute myeloid leukemia and pediatric acute lymphoblastic leukemias. NK cell is mediated by donor NK cells recovering very early after transplant. The self donor KIR ligands educates the donor NK repertoire and arms functional NK cells which express inhibitory killer cell immunoglobulin-like receptor(s) (KIRs) for self-class I ligand(s), They sense missing expression of donor KIR ligand(s) in the recipient and mediate alloreactivity. Donor-versus-recipient NK cell alloreactivity is evaluated by KIR genotyping and phenotyping and functional assay.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ruggeri L, Capanni M, Urbani E et al (2002) Effectiveness of donor natural killer cell alloreactivity in mismatched hematopoietic transplants. Science 295:2097–20100
Ruggeri L, Capanni M, Casucci M et al (1999) Role of natural killer cell alloreactivity in HLAmismatched hematopoietic stem cell transplantation. Blood 94:333–339
Pende D, Marcenaro S, Falco M et al (2009) Anti-leukemia activity of alloreactive NK cells in KIR ligand-mismatched haploidentical HSCT for pediatric patients: evaluation of the functional role of activating KIR and redefinition of inhibitory KIR specificity. Blood 113:3119–3129
Willemze R et al (2009) KIR-ligand incompatibility in the graft-versus-host direction improves outcomes after umbilical cord blood transplantation for acute leukemia. Leukemia 23:492–500
Ciccone E, Pende D, Viale O et al (1992) Evidence of a natural killer (NK) cell repertoire for (allo) antigen recognition: definition of five distinct NK-determined allospecificities in humans. J Exp Med 175:709–718
Colonna M, Brooks EG, Falco M et al (1993) Generation of allospecific natural killer cells by stimulation across a polymorphism of HLA-C. Science 260:1121–1124
Foley BA, De Santis D, Van Beelen E et al (2008) The reactivity of Bw4+ HLA-B and HLA-A alleles with KIR3DL1: implications for patient and donor suitability for haploidentical stem cell transplantations. Blood 112:435–443
Velardi A (2008) Role of KIRs and KIR ligands in hematopoietic transplantation. Curr Opin Immunol 20:581–587
Velardi A, Ruggeri L, Mancusi A et al (2009) Natural killer cell allorecognition of missing self in allogeneic hematopoietic transplantation: a tool for immunotherapy of leukemia. Curr Opin Immunol 21:525–530
Foley B, De Santis D, Lathbury L et al (2008) KIR2DS1-mediated activation overrides NKG2A-mediated inhibition in HLA-C C2- negative individuals. Int Immunol 20:555–563
Chewning JH, Gudme CN, Hsu KC et al (2007) KIR2DS1-positive NK cells mediate alloresponse against the C2 HLA-KIR ligand group in vitro. J Immunol 179:854–868
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Ruggeri, L., Mancusi, A., Urbani, E., Velardi, A. (2016). Identifying NK Alloreactive Donors for Haploidentical Hematopoietic Stem Cell Transplantation. In: Bondanza, A., Casucci, M. (eds) Tumor Immunology. Methods in Molecular Biology, vol 1393. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3338-9_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3338-9_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3336-5
Online ISBN: 978-1-4939-3338-9
eBook Packages: Springer Protocols