Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition pp 103-112 | Cite as
In Vitro Regeneration of Endangered Medicinal Plant Heliotropium kotschyi (Ramram)
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Abstract
Heliotropium kotschyi (Ramram) is an important endangered medicinal plant distributed in the Kingdom of Bahrain. Plant tissue culture technique is applied for ex situ conservation study. Nodal stem segments are cultured in modified MS media supplemented with various combination and concentration of plant growth regulators (PGRs). Plants are regenerated via shoot organogenesis from the nodal meristems. Plants are regenerated in three different steps: initial shoot development, shoot multiplication, and rooting. After 4 weeks of culture, 100 % explants respond to shoot initiation on the medium containing 8.88 μM BAP and 5.71 μM IAA. The highest frequency of shoot regeneration is observed in the same media after second subculture of shoots. The highest rooting frequency is observed in the presence of 2.85 μM IAA. After root development, the plantlets are transferred to pots filled with soil and 60 % of plants survived after 45 days. This plant regeneration protocol is of great value for rapid desert plant propagation program.
Key words
Endangered Ex situ conservation Heliotropium kotschyi Organogenesis Plant regenerationNotes
Acknowledgements
The work was supported by College of Graduate Studies, Desert and Arid Zone Sciences Program, Arabian Gulf University, Manama, Kingdom of Bahrain.
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