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Applications of Imaging Flow Cytometry for Microalgae

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Book cover Imaging Flow Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique aspect to analyses, and can enable correlation between molecular phenomena resulting in alterations in cellular phenotype. Unicellular microalgae are amenable to high-throughput analysis to capture the diversity of cell types in natural samples, or diverse cellular responses in clonal populations, especially using imaging cytometry. Using examples from our laboratory, we review applications of imaging cytometry, specifically using an Amnis® ImageStream®X instrument, to characterize photosynthetic microalgae. Some of these examples highlight advantages of imaging flow cytometry for certain research objectives, but we also include examples that would not necessarily require imaging and could be performed on a conventional cytometer to demonstrate other concepts in cytometric evaluation of microalgae. We demonstrate the value of these approaches for (1) analysis of populations, (2) documentation of cellular features, and (3) analysis of gene expression.

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Author information

Authors and Affiliations

  1. Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA

    Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Jesse C. Traller, Roshan P. Shrestha, Orna Cook, Eva L. Sánchez-Alvarez & Kalpana Manandhar-Shrestha

  2. Amnis part of EMD Millipore, Seattle, WA, USA

    Haley R. Pugsley & Benjamin Alderete

  3. Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, USA

    Sarah R. Smith

Authors
  1. Mark Hildebrand
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  2. Aubrey Davis
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  3. Raffaela Abbriano
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  4. Haley R. Pugsley
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  5. Jesse C. Traller
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  6. Sarah R. Smith
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  7. Roshan P. Shrestha
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  8. Orna Cook
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  9. Eva L. Sánchez-Alvarez
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  10. Kalpana Manandhar-Shrestha
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  11. Benjamin Alderete
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Corresponding author

Correspondence to Mark Hildebrand .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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Hildebrand, M. et al. (2016). Applications of Imaging Flow Cytometry for Microalgae. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

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