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A Transgenic Mouse Model of Poliomyelitis

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Poliovirus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1387))

Abstract

Transgenic mice (tg mice) that express the human poliovirus receptor (PVR), CD155, are susceptible to poliovirus and develop a neurological disease that resembles human poliomyelitis. Assessment of the neurovirulence levels of poliovirus strains, including mutant viruses produced by reverse genetics, circulating vaccine-derived poliovirus, and vaccine candidates, is useful for basic research of poliovirus pathogenicity, the surveillance of circulating polioviruses, and the quality control of oral live poliovirus vaccines, and does not require the use of monkeys. Furthermore, PVR-tg mice are useful for studying poliovirus tissue tropism and host immune responses. PVR-tg mice can be bred with mice deficient in the genes involved in viral pathogenicity. This report describes the methods used to analyze the pathogenicity and immune responses of poliovirus using the PVR-tg mouse model.

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Author information

Authors and Affiliations

  1. Neurovirology Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan

    Satoshi Koike

  2. National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo, 208-0011, Japan

    Noriyo Nagata

Authors
  1. Satoshi Koike
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  2. Noriyo Nagata
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Correspondence to Satoshi Koike .

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Editors and Affiliations

  1. Division of Virology, NIBSC, Hertfordshire, United Kingdom

    Javier Martín

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Koike, S., Nagata, N. (2016). A Transgenic Mouse Model of Poliomyelitis. In: Martín, J. (eds) Poliovirus. Methods in Molecular Biology, vol 1387. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3292-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-3292-4_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3291-7

  • Online ISBN: 978-1-4939-3292-4

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