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Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

  • Pooja Saxena
  • Eva C. Thuenemann
  • Frank Sainsbury
  • George P. LomonossoffEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1385)

Abstract

This chapter constitutes a practical guide to using the “pEAQ” vector series for transient or stable expression of one or more protein(s) in Nicotiana benthamiana plants. The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-HT, is used. The expression vector pEAQ-HT combines the user-friendly pEAQ plasmid with CPMV-HT to provide a system for high-level expression of proteins in plants.

Keywords

Transient expression Agro-infiltration Viral vectors pEAQ CPMV-HT 

Notes

Acknowledgement

This work was supported by grant BB/J004561/1 from the Biotechnology and Biological Research Council (BBSRC) UK and the John Innes Foundation.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Pooja Saxena
    • 1
  • Eva C. Thuenemann
    • 2
  • Frank Sainsbury
    • 3
  • George P. Lomonossoff
    • 2
    Email author
  1. 1.Department of ChemistryIndiana UniversityBloomingtonUSA
  2. 2.Department of Biological ChemistryJohn Innes CentreNorwichUK
  3. 3.Australian Institute for Bioengineering and Nanotechnology (AIBN)The University of QueenslandSt LuciaAustralia

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