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Recombinant Proteins from Plants pp 259–270Cite as

A Standardized Lepidopteran Bioassay to Investigate the Bioactivity of Insecticidal Proteins Produced in Transgenic Crops

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1385))

Abstract

Insecticidal bioassays are the only reliable method to investigate the biological activity of an insecticidal protein and therefore provide an essential toolkit for the characterization and potency determination of these proteins. Here we present a standardized method for a lepidopteran larval bioassay, which is optimized to specifically estimate activity of insecticidal proteins produced in transgenic plants. The treatment can be either applied to the surface of the artificial diet, or blended into the diet.

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Author information

Authors and Affiliations

  1. Syngenta Crop Protection, LLC., 3054 E. Cornwallis Road, 12257, Research Triangle Park, NC, 27709, USA

    Gerson Graser & Frederick S. Walters

Authors
  1. Gerson Graser
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  2. Frederick S. Walters
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Corresponding author

Correspondence to Gerson Graser .

Editor information

Editors and Affiliations

  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario, Canada

    Jacqueline MacDonald

  2. Department of Biology, Western University, London, Ontario, Canada

    Igor Kolotilin

  3. London, Ontario, Canada

    Rima Menassa

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Graser, G., Walters, F.S. (2016). A Standardized Lepidopteran Bioassay to Investigate the Bioactivity of Insecticidal Proteins Produced in Transgenic Crops. In: MacDonald, J., Kolotilin, I., Menassa, R. (eds) Recombinant Proteins from Plants. Methods in Molecular Biology, vol 1385. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3289-4_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3289-4_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3288-7

  • Online ISBN: 978-1-4939-3289-4

  • eBook Packages: Springer Protocols

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