Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor
Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficient downstream process for antibody recovery from the viscous spent culture medium using expanded bed adsorption (EBA) chromatography.
KeywordsAntibody purification Expanded bed chromatography Nicotiana tabacum Bright Yellow 2 (BY-2) Plant cell suspension culture Scaled-up manufacture Single-use technology
This work was supported by the European Union Seventh Framework Program under grant agreement no. 227420 (CoMoFarm).
- 16.Eibl R, Eibl D (2006) Design and use of the wave bioreactor for plant cell culture. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, Netherlands, pp 203–227Google Scholar
- 19.Zhang X, Stettler M, De Sanctis D et al (2010) Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale. Adv Biochem Eng Biotechnol 115:33–53Google Scholar