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Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology

  • Johanna Gottschamel
  • Andreas LösslEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1385)

Abstract

Plastid transformation for the expression of recombinant proteins and entire enzymatic pathways has become a promising tool for plant biotechnology in the past decade. Several improvements of the technology have turned plant plastids into robust and dependable expression platforms for multiple high value compounds. In this chapter, we describe our current methodology based on Gateway® recombinant cloning, which we have adapted for plastid transformation. We describe the steps required for cloning, biolistic transformation, identification, and regeneration of transplastomic plant lines and Western blot analysis.

Keywords

Plastid transformation Gateway® recombinant cloning technology Biolistic transformation Southern blot analysis Western blot analysis 

Notes

Acknowledgements

This work was supported by the GLOBVAC program (Project 192510), the Research Council of Norway (RCN), Bioforsk.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Crop SciencesUniversity of Natural Resources and Life SciencesTulln a. d. DonauAustria

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