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Run-Up and Run-Down

  • Protocol
Book cover Electrophysiological Analysis of Synaptic Transmission

Part of the book series: Neuromethods ((NM,volume 112))

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Abstract

A burden associated with electrophysiology is potential artifacts that look like physiological events, but are instead mistakenly incorporated into the experimental recordings. Two such artifacts discussed in this chapter are current run-up and current run-down. Simply put, baseline responses increase (run-up) or decrease (run-down) over time. Membrane channels recorded using cell-attached, whole-cell, and excised-patch configurations are all susceptible to run-up/run-down. The purpose of this chapter is to discuss empirically derived causes of run-up and run-down so that both errors can be either identified or avoided before or during current measurements. Unfortunately, data showing causes and solutions to run-up and run-down in all membrane channels does not exist. However, we discuss receptors and cell types that have been studied for the purpose of keeping the experimenter aware of problem solving strategies during electrophysiological measurements.

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Authors and Affiliations

  1. Neuroscience Department, University of Pittsburgh, Langley Hall, Pittsburgh, PA, 15260, USA

    Nicholas Graziane & Yan Dong

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  1. Nicholas Graziane
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  2. Yan Dong
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Correspondence to Nicholas Graziane .

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Graziane, N., Dong, Y. (2016). Run-Up and Run-Down. In: Electrophysiological Analysis of Synaptic Transmission. Neuromethods, vol 112. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3274-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3274-0_16

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3273-3

  • Online ISBN: 978-1-4939-3274-0

  • eBook Packages: Springer Protocols

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