• Nicholas GrazianeEmail author
  • Yan Dong
Part of the Neuromethods book series (NM, volume 112)


Amplitudes in in vitro electrophysiological measurements refer to the value of currents or potentials recorded from one cell or a population of cells at a given time point. This chapter discusses amplitude measurements for in vitro slice electrophysiology and covers appropriate calculations for action potential amplitudes, field potential amplitudes, postsynaptic current/potential amplitudes, and steady-state current amplitudes. Like many electrophysiological measurements, technical considerations for precise calculations are important. These technical considerations are addressed at the end of this chapter.

Key words

Action potentials Local field potentials Synaptic currents/potentials 


  1. 1.
    Baranauskas G, Tkatch T, Nagata K, Yeh JZ, Surmeier DJ (2003) Kv3.4 subunits enhance the repolarizing efficiency of Kv3.1 channels in fast-spiking neurons. Nat Neurosci 6(3):258–266CrossRefPubMedGoogle Scholar
  2. 2.
    Bean BP (2007) The action potential in mammalian central neurons. Nat Rev Neurosci 8(6):451–465CrossRefPubMedGoogle Scholar
  3. 3.
    Du J, Zhang L, Weiser M, Rudy B, McBain CJ (1996) Developmental expression and functional characterization of the potassium-channel subunit Kv3.1b in parvalbumin-containing interneurons of the rat hippocampus. J Neurosci 16(2):506–518PubMedGoogle Scholar
  4. 4.
    Erisir A, Lau D, Rudy B, Leonard CS (1999) Function of specific K(+) channels in sustained high-frequency firing of fast-spiking neocortical interneurons. J Neurophysiol 82(5):2476–2489PubMedGoogle Scholar
  5. 5.
    Lien CC, Martina M, Schultz JH, Ehmke H, Jonas P (2002) Gating, modulation and subunit composition of voltage-gated K(+) channels in dendritic inhibitory interneurones of rat hippocampus. J Physiol 538(Pt 2):405–419CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Martina M, Schultz JH, Ehmke H, Monyer H, Jonas P (1998) Functional and molecular differences between voltage-gated K+ channels of fast-spiking interneurons and pyramidal neurons of rat hippocampus. J Neurosci 18(20):8111–8125PubMedGoogle Scholar
  7. 7.
    Massengill JL, Smith MA, Son DI, O’Dowd DK (1997) Differential expression of K4-AP currents and Kv3.1 potassium channel transcripts in cortical neurons that develop distinct firing phenotypes. J Neurosci 17(9):3136–3147PubMedGoogle Scholar
  8. 8.
    Rudy B, McBain CJ (2001) Kv3 channels: voltage-gated K+ channels designed for high-frequency repetitive firing. Trends Neurosci 24(9):517–526CrossRefPubMedGoogle Scholar
  9. 9.
    Faber ES, Sah P (2002) Physiological role of calcium-activated potassium currents in the rat lateral amygdala. J Neurosci 22(5):1618–1628PubMedGoogle Scholar
  10. 10.
    Sah P (1996) Ca(2+)-activated K+ currents in neurones: types, physiological roles and modulation. Trends Neurosci 19(4):150–154CrossRefPubMedGoogle Scholar
  11. 11.
    Sah P, Faber ES (2002) Channels underlying neuronal calcium-activated potassium currents. Prog Neurobiol 66(5):345–353CrossRefPubMedGoogle Scholar
  12. 12.
    Sah P, McLachlan EM (1992) Potassium currents contributing to action potential repolarization and the afterhyperpolarization in rat vagal motoneurons. J Neurophysiol 68(5):1834–1841PubMedGoogle Scholar
  13. 13.
    Shao LR, Halvorsrud R, Borg-Graham L, Storm JF (1999) The role of BK-type Ca2+-dependent K+ channels in spike broadening during repetitive firing in rat hippocampal pyramidal cells. J Physiol 521(Pt 1):135–146CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Nicoll RA, Schmitz D (2005) Synaptic plasticity at hippocampal mossy fibre synapses. Nat Rev Neurosci 6(11):863–876CrossRefPubMedGoogle Scholar
  15. 15.
    Johnston D, Wu SMS (1995) Foundations of cellular neurophysiology. MIT Press, Cambridge, MAGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Neuroscience DepartmentUniversity of PittsburghPittsburghUSA

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