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A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan

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Clinical Applications of Mass Spectrometry in Drug Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1383))

Abstract

Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of <10 %.

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Correspondence to Uttam Garg Ph.D. .

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Deng, S., Kiscoan, M., Frazee, C., Abdel-Rahman, S., Dalal, J., Garg, U. (2016). A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan. In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 1383. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3252-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3252-8_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3251-1

  • Online ISBN: 978-1-4939-3252-8

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