Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry
Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule—RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.
Key wordsRNA Aptamer Small molecule ITC Kd In vitro transcription
This work was supported by the Deutsche Forschungsgemeinschaft (SFB902 A2) and EU FP7-KBBE-2013-7 no. 613745, Promys. We thank Katharina Keim for critical reading the manuscript.