• Alessandro Pinto
  • Pedro Nadal Polo
  • Miriam Jauest Rubio
  • Marketa Svobodova
  • Teresa Mairal Lerga
  • Ciara K. O’SullivanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1380)


Real-time Apta-PCR is a methodology that can be used for a wide variety of applications ranging from food quality control to clinical diagnostics. This method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers. Ultra-low detection of target analyte can potentially be achieved, or, improved detection limits can be achieved with aptamers of low-medium affinity. Herein, we describe a generic methodology coined real-time Apta-PCR, using a model target (β-conglutin) and a competitive format, which can be adapted for the detection of any target which an aptamer has been selected for.

Key words

Apta-PCR Aptamer Protein quantification 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Alessandro Pinto
    • 1
    • 2
  • Pedro Nadal Polo
    • 1
    • 3
  • Miriam Jauest Rubio
    • 1
  • Marketa Svobodova
    • 1
  • Teresa Mairal Lerga
    • 1
  • Ciara K. O’Sullivan
    • 1
    • 4
    Email author
  1. 1.Department of Chemical EngineeringUniversitat Rovira I VirgiliTarragonaSpain
  2. 2.Department of BioengineeringRice UniversityHoustonUSA
  3. 3.Center for Omic Sciences (COS)Universitat Rovira I VirgiliReusSpain
  4. 4.Institució Catalana de Recerca I Estudis AvançatsBarcelonaSpain

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