Abstract
Fluorescence imaging techniques could be used in different ways to study the interaction of aptamers with biological systems from cell culture to animal models. Here, we present the methods developed in our laboratory for fluorescently labeled aptamers, study their internalization inside living cells using time-lapse microscopy, and monitor their biodistribution in mice bearing subcutaneous xenograft tumors using planar fluorescence imaging and fluorescence diffuse optical tomography (fDOT).
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Acknowledgements
We are grateful to Dr. Rui Sousa (University of Texas, San Antonio) for his generous gift of a T7Y639F RNA polymerase-expressing plasmid and to Carine Pestourie, Agnès Cibiel, Benoit Jego, Isabelle Janssens, Daniel Miotto Dupont, Anikitos Garofalakis, and Bertrand Tavitian for their work on aptamer imaging in our laboratory. Studies relating to in vivo imaging of aptamers in our laboratories were supported by grants from the “Agence Nationale pour la Recherche” [projects ANR-RNTS TomoFluo3D, ANR-PNANO nanorings and under the frame of EuroNanoMed (project META)]; the FMT -XCT European program [Grant agreement no. 201792]; and the European Molecular Imaging Laboratory (EMIL) network [EU contract LSH-2004-503569].
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Théodorou, I., Quang, N.N., Gombert, K., Thézé, B., Lelandais, B., Ducongé, F. (2016). In Vitro and In Vivo Imaging of Fluorescent Aptamers. In: Mayer, G. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 1380. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3197-2_11
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DOI: https://doi.org/10.1007/978-1-4939-3197-2_11
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