Abstract
Aptamers compete with antibodies in many applications, in which high-affinity and specificity ligands are needed. In this regard, fluorescence-tagged aptamers have gained applications in flow and imaging cytometry for detecting cells expressing distinct antigens. Here we present prospective methods, as a starting point, for using these high-affinity ligands for cytometry applications.
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Acknowledgments
This work was supported by Thematic Grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, project No. 2012/50880-4 to H.U. and No. 2013/17368-0 to J.E.K.) and by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, project No. 486294/2012- 9 and 467465/2014-2 to H.U.), Brazil. V.B. acknowlegdes postdoctoral fellowship support by FAPESP (project No. 2011/19678-1).
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Nascimento, I.C., Nery, A.A., Bassaneze, V., Krieger, J.E., Ulrich, H. (2016). Applications of Aptamers in Flow and Imaging Cytometry. In: Mayer, G. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 1380. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3197-2_10
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DOI: https://doi.org/10.1007/978-1-4939-3197-2_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3196-5
Online ISBN: 978-1-4939-3197-2
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