1H NMR Metabolomic Footprinting Analysis for the In Vitro Screening of Potential Chemopreventive Agents
Metabolomics is the quantification and analysis of the concentration profiles of low-molecular-weight compounds present in biological samples. In particular metabolic footprinting analysis, based on the monitoring of metabolites consumed from and secreted into the growth medium, is a valuable tool for the study of pharmacological and toxicological effects of drugs. Mass spectrometry and nuclear magnetic resonance (NMR) are the two main complementary techniques used in this field. Although less sensitive, NMR gives a direct fingerprint of the system, and the spectra obtained contain metabolic information that can be distilled by chemometric techniques.
In this chapter, we present how metabolomic footprinting can be used to assess in vitro a potential chemopreventive molecule as metformin.
Key wordsChemoprevention Metabolomics Footprinting analysis NMR spectroscopy Principal component analysis Euclidean distance
We thank Prof. Cesare Manetti, supervisor of NMR Laboratory of Chemistry Dpt., “Sapienza” University of Rome. We thank all the members of Dr. Giovanni Blandino’s and Dr. Sabrina Strano’s laboratories, Italian National Cancer Institute “Regina Elena,” Rome, Italy, for many years of collaboration in the metformin project. We are grateful to Dr. Alessandro Giuliani, Dpt. of Environment and Primary Prevention, Istituto Superiore di Sanità, Rome, Italy, for his useful comments and suggestions on the data analysis.