Application of RNA-Seq Technology in Cancer Chemoprevention

  • Frauke GoemanEmail author
  • Maurizio Fanciulli
Part of the Methods in Molecular Biology book series (MIMB, volume 1379)


RNA-sequencing is a revolutionary tool to follow differential expression after treatment with cancer chemopreventive agents. It allows a real genome-wide screening independent of prior assumptions and is well suited for analyzing coding but also long noncoding RNAs. It still consents the discovery of new genes and isoforms and increased our knowledge of antisense and other noncoding RNAs in a tremendous manner. Moreover, it permits to detect low-abundance and biologically critical isoforms and reveals genetic variants and gene fusions in one single assay. Here, we provide a detailed protocol for stranded RNA-sequencing.

Key words

RNA-Seq Deep-sequencing Chemoprevention Transcriptome profiling Long noncoding RNA Stranded RNA-Seq Transcriptional signature Novel transcript and isoform discovery 



We greatly appreciate the support given by the Progetto Finalizzato Ministero della Salute “Tumori Femminili.”


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Translational Oncogenomics UnitItalian National Cancer Institute “Regina Elena”RomeItaly
  2. 2.Laboratory of Epigenetic, Molecular Medicine AreaItalian National Cancer Institute “Regina Elena”RomeItaly

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