Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women

  • Sara Donzelli
  • Giovanni Blandino
  • Paola MutiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1379)


MicroRNAs are key regulators of different biological processes and their deregulation is associated with the occurrence of many diseases among which cancer. Due to the higher stability of microRNAs and to the easiness in their detection both in organs than in biological fluids, many studies are turned toward potential use of this small molecules as biomarkers for the prediction and diagnosis of different types of cancer. Here we describe the experiment protocol that we have used for microRNA profiling analysis in buffy coat samples of women who developed breast cancer versus women who remained healthy during a 20 year follow-up period, with the aim to identify predictive microRNAs of breast cancer occurrence.

Key words

microRNA microRNA profiling Buffy coat Breast cancer RNA extraction 



We are indebted to the 10,786 ORDET participants. We also thank Dr. Paolo Contiero and the staff of the Lombardy Cancer Registry for technical assistance. This work was supported by Department of Defense grant W81 XWH 04 1 0195 and by the Veronesi Foundation.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Translational Oncogenomics UnitRegina Elena Italian National Cancer InstituteRomeItaly
  2. 2.Laboratory of Translational Oncogenomics, Molecular Medicine DepartmentRegina Elena National Cancer InstituteRomeItaly
  3. 3.Department of Oncology, Faculty of Health ScienceMcMaster UniversityHamiltonCanada

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