Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women
MicroRNAs are key regulators of different biological processes and their deregulation is associated with the occurrence of many diseases among which cancer. Due to the higher stability of microRNAs and to the easiness in their detection both in organs than in biological fluids, many studies are turned toward potential use of this small molecules as biomarkers for the prediction and diagnosis of different types of cancer. Here we describe the experiment protocol that we have used for microRNA profiling analysis in buffy coat samples of women who developed breast cancer versus women who remained healthy during a 20 year follow-up period, with the aim to identify predictive microRNAs of breast cancer occurrence.
Key wordsmicroRNA microRNA profiling Buffy coat Breast cancer RNA extraction
We are indebted to the 10,786 ORDET participants. We also thank Dr. Paolo Contiero and the staff of the Lombardy Cancer Registry for technical assistance. This work was supported by Department of Defense grant W81 XWH 04 1 0195 and by the Veronesi Foundation.
- 16.Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nat Protoc 1:581–58517Google Scholar
- 17.Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate: A practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B. Methodological, 57:289–300Google Scholar