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South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity

  • Zodwa DlaminiEmail author
  • Zukile Mbita
  • David Bates
Part of the Methods in Molecular Biology book series (MIMB, volume 1379)

Abstract

RT-PCR is an invaluable tool for the detection and characterization of mRNA. Cancer cell lines are treated with crude plant extracts and RNA is extracted and purified with DNase prior to RT-PCR. RT-PCR first-strand cDNA synthesis is done using random primers and can be refrigerated at 4 °C. PCR from the stored cDNA is performed using transcript-specific primers and electrophoresed on a molecular grade agarose gel to separate the splice variants.

Key words

Anticancer splicing activity Herbal plant extracts RNA extraction First-strand cDNA synthesis DNase treatment Agarose gel electrophoresis 

Notes

Acknowledgement

The British Royal Society, the South African Medical Research Council, the Oppenheimer Trust, and the National Research Foundation of South Africa supported this work.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Research, Innovation & EngagementsMangosuthu University of TechnologyDurbanSouth Africa
  2. 2.Mangosuthu University of Technology Research, Innovation and Engagements PortfolioDurbanSouth Africa
  3. 3.Faculty of Medicine and Health Sciences, Division of Pre-clinical OncologyUniversity of NottinghamNottinghamUK

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