3D Tumor Models and Time-Lapse Analysis by Multidimensional Microscopy
The 3D culture is advantageous in reflecting the in vivo condition compared to the 2D culture; however, imaging 3D-cultured cells may be a challenge due to technical restrictions. Recent development of confocal spinning disc microscope system as well as sophisticated software has enabled us to monitor dynamism of cell movement in multiple dimensions. Here we describe the method for time-lapse imaging of 3D-cultured cancer cells co-cultured with non-cancerous cells and discuss current limitations and future perspectives.
Key wordsThree-dimensional culture Time-lapse imaging Multidimensional microscopy Live cell imaging Deconvolution
We thank K. Welzel, L. Ivers, and F. Owolabi for their support and acquisition of images. This work was funded by University College Dublin Core Funding to NI.
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