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3D Tumor Models and Time-Lapse Analysis by Multidimensional Microscopy

  • Dimitri Scholz
  • Nobue ItasakiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1379)

Abstract

The 3D culture is advantageous in reflecting the in vivo condition compared to the 2D culture; however, imaging 3D-cultured cells may be a challenge due to technical restrictions. Recent development of confocal spinning disc microscope system as well as sophisticated software has enabled us to monitor dynamism of cell movement in multiple dimensions. Here we describe the method for time-lapse imaging of 3D-cultured cancer cells co-cultured with non-cancerous cells and discuss current limitations and future perspectives.

Key words

Three-dimensional culture Time-lapse imaging Multidimensional microscopy Live cell imaging Deconvolution 

Notes

Acknowledgements

We thank K. Welzel, L. Ivers, and F. Owolabi for their support and acquisition of images. This work was funded by University College Dublin Core Funding to NI.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Conway InstituteUniversity College DublinDublinIreland
  2. 2.School of Medicine and Medical ScienceUniversity College DublinDublinIreland
  3. 3.Faculty of Health SciencesUniversity of BristolBristolUK

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